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     33     <h1>
     34      Cold agglutinin disease: fish out of water?
     35     </h1>
     36     <p>
     37      <time id="post-date">2024-03-22</time>
     38     </p>
     39     <p id="post-excerpt">
     40      Cold agglutinin disease is a fascinating and strange phenomenon, and might happen because we're fish.
     41     </p>
     42     <h2>
     43      CAD: a matter of degree
     44     </h2>
     45     <p>
     46      CAD is a disease of the cells that make the immune system, in which
     47 they overproduce a protein called cold agglutinin, resulting in a
     48 cascade of unfortunate events that are typically triggered by the blood
     49 getting slightly too cold (going outside in the winter, getting ice
     50 cream out of the freezer, drinking a Slurpie, etc.). Red blood cells
     51 clump together and cause painful, blue fingers/toes/etc., sometimes so
     52 severe the affected bits die and fall off, and an autoimmune attack on
     53 the red blood cells begins, resulting in some of them being eaten alive
     54 by the liver and spleen and others being (literally) exploded while
     55 still in the blood vessels.
     56     </p>
     57     <p>
     58      Everybody has cold agglutinins, these proteins that cause the
     59 clumping (“agglutination”), at some low level. They’re a type of IgM, a
     60 class of large proteins that are a key part of the immune system. IgM is
     61 really good at sticking things together, which is exactly what you want
     62 to happen in certain infections, etc., but which happens aberrantly, and
     63 sometimes dramatically, with red blood cells in CAD.
     64     </p>
     65     <p>
     66      All red blood will agglutinate at 0-5C. This has been known since, at
     67 the latest, 1903. Karl Landsteiner figured it out. He’s the guy who won
     68 the Nobel Prize for discovering blood types, which was based on
     69 experiments with “iso-agglutination.” With blood typing came the ability
     70 to cross-match blood for safe transfusion, which resulted in a huge leap
     71 forward in our ability to help people who lose a lot of it, as in
     72 surgery and trauma. We think of Landsteiner as the blood typing dude,
     73 but it’s probably more accurate and expansive to think of him as the
     74 dude who first exhaustively characterized the different situations in
     75 which blood agglutinates.
     76     </p>
     77     <p>
     78      Landsteiner’s discoveries came just in time for the World Wars, and
     79 folks got to collecting blood in earnest, on a massive scale. We went
     80 from a few laboratory fridges with dozens or hundreds of blood samples
     81 for experiments, to blood banks worldwide, collectively full of hundreds
     82 of thousands of units of blood for use in the operating room and
     83 battlefield. Some oddities about human existence are only found when the
     84 numbers grow to this size: blood bank technicians found that some blood
     85 agglutinates all the way up to 25C (~77F, “room temperature”). This
     86 resulted in difficulty with accurately typing blood, which led to a
     87 number of deaths.
     88     </p>
     89     <p>
     90      In 1946 Lubinski and Goldbloom at Johns Hopkins published a paper
     91 describing seven patients with blood that would agglutinate all the way
     92 up to 37C (98.6F). All of these patients had brisk hemolysis (red blood
     93 cell explosion).
     94     </p>
     95     <p>
     96      Putting it together, we have three categories of blood agglutination
     97 in response to temperature: everyone’s blood will agglutinate in a
     98 freezer, a small but significant portion of people have blood that
     99 agglutinates at room temperature, and there are an unfortunate few who
    100 have blood that agglutinates while still (relatively) warm in their
    101 bodies. In all of these cases, it’s a cold agglutinin, a certain IgM
    102 protein, that coordinates the clumping.
    103     </p>
    104     <p>
    105      As a doctor I can accept that sometimes the body does horrible
    106 things, about which the most that can be fairly said, despite all that
    107 science can provide, is that they are random. A person’s blood deciding
    108 to turn on them at the slightest cold provocation is well within the
    109 realm of crazy things we deal with on a daily basis. I would love to
    110 understand why everything horrible happens, but often have to move
    111 forward only knowing that it does happen, and hope that there might be a
    112 thing or two I can do to offset the horribleness.
    113     </p>
    114     <p>
    115      I can also accept that unnatural environments lead to unnatural
    116 phenomena, such as blood clumping in a freezer. That’s a
    117 physical/chemical situation that never happens in a living animal (at
    118 least, in vertebrates. Don’t get me started on the Antarctic midge).
    119 There doesn’t need to be an evolutionary justification for the clumping
    120 in this case, just a biochemical one.
    121     </p>
    122     <p>
    123      It’s the room temperature thing that bothers me. In physiologic
    124 findings that are so clearly a matter of degree, with an obvious sliding
    125 scale, I wonder: what in our deep past created the affordance for this
    126 thing in the first place? Why do we have cold agglutinins at all?
    127     </p>
    128     <h2>
    129      Whence cold agglutinins? Probably fish. And fish are us.
    130     </h2>
    131     <p>
    132      Sigbjorn Berentsen is a Norwegian physician and researcher (CAD is,
    133 as you might expect, much more common in colder climates), and is The
    134 Man when it comes to understanding and treating CAD in the modern
    135 era.
    136     </p>
    137     <p>
    138      A recent paper from him had this to say about the most likely
    139 possibility of the origin of cold agglutinins:
    140     </p>
    141     <blockquote>
    142      <p>
    143       …the physiological function of CAs has not been clarified. It is
    144 difficult to envision a functional role of antibodies with a temperature
    145 optimum way below body temperature. Comparative studies, however, have
    146 strongly indicated that the evolution of the adaptive immune system
    147 began with the jawed vertebrates. Cartilaginous fish, which are
    148 phylogenetically ancient and considered closely related to the first
    149 jawed vertebrates, have only one immunoglobulin class in common with
    150 humans: IgM… [T]he temperature optimum of CAs is much closer to the
    151 environmental and body temperature of non-mammal sea vertebrates.
    152 Furthermore, CAs can react with antigens other than RBC surface
    153 macromolecules, and structures closely related to the I antigen are
    154 present on some microorganisms such as Streptococcus and Listeria
    155 species. Thus, one might explain human CAs as remnants of a primitive
    156 vertebrate immune system.
    157      </p>
    158     </blockquote>
    159     <p>
    160      <a href="https://doi.org/10.3389/fimmu.2020.00590">https://doi.org/10.3389/fimmu.2020.00590</a>
    161     </p>
    162     <p>
    163      So, ancient fish, swimming in room temperature or colder water, had
    164 to fight certain bacteria. The fish are us, if you go back far enough.
    165 We happened to have held on to this ability, these proteins, an
    166 immunologic vestigial tail. Certain proteins on our red blood cells look
    167 an awful lot like the proteins on those bacteria, and, if you are
    168 unlucky enough that your vestigial tail grows out more than the average
    169 bear, friendly fire ensues. (That last sentence has a staggering number
    170 of mixed metaphors. Smiling, tongue firmly in cheek, he turned to you
    171 and said, “Humans <em>are</em> mixed metaphors.”)
    172     </p>
    173     <h2>
    174      Conclusion, prefaced by an aside on Jaron Lanier
    175     </h2>
    176     <p>
    177      Jaron Lanier is a computer scientist, ethnomusicologist, and
    178 all-around wonderful weirdo who was behind a lot of the early virtual
    179 reality experiments in the 70s-80s (and now works on VR with Microsoft
    180 when he’s not putting on concerts showcasing Chinese mouth-organs). He
    181 would do this thing where he would create VR avatars with many more
    182 limbs than humans (lobster is a classic one) and attach sensors to the
    183 participants to allow them to control all the limbs with various subtle
    184 movements (hips, elbows, knees, etc.). They found that it didn’t take
    185 long for the humans to achieve surprising proficiency acting as a
    186 &gt;4-limbed creature, and he would wax poetic about the ancient
    187 phylogenetic compatibility still hidden in the motor centers of the
    188 brain, and other related, delicious ideas (maybe it’s not four limbs and
    189 20 digits that are mapped discretely and <em>a priori</em> into the
    190 human brain, maybe it’s the capacity to map any number of prehensile
    191 bits that is inherent, etc. The therapeutic and geeky possibilities
    192 leading from this are way too much fun to contain in an aside).
    193     </p>
    194     <p>
    195      Anyway, the point it this: I love it when the answer is, “idk, maybe
    196 we’re fish. What’s a fish, anyway?”
    197     </p>
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